Pest Management Science (2019) 75, 234-245

Zhiqiang Tian, Guisheng Qiu, Yanyan Li, Huaijiang Zhang, Wentao Yan, Qiang Yue and Lina Sun (2019)
Molecular characterization and functional analysis of pheromone binding proteins and general odorant binding proteins from Carposina sasakii Matsumura (Lepidoptera: Carposinidae)
Pest Management Science 75 (1), 234-245
Abstract:
BACKGROUND
The peach fruit moth, Carposina sasakii Matsumura (Lepidoptera: Carposinidae), is one of the most destructive pests of pome and stone fruits, while few studies of their molecular biology and physiology have been conducted. Research into CsasPBPs (Carposina sasakii pheromone binding proteins) and CsasGOBPs (Carposina sasakii general odorant binding proteins) may provide insights in to the mechanisms of olfaction in Carposina sasakii.
RESULTS
In our study, results of real time quantitative polymerase chain reaction (qPCR) assays demonstrated that CsasPBP1–3 and CsasGOBP1–2 transcripts were abundantly expressed in the antennae of both sexes, suggesting they play a vital role in olfaction. In addition, to examine specific functional differences between pheromone binding proteins (PBPs) and general odorant binding proteins (GOBPs), fluorescence competitive binding assays were used to measured the binding affinities for the two sex pheromones and 18 apple plant volatiles. As a result, both PBPs and GOBPs showed stronger binding affinities to Z-7-eicosene-11-one than Z-7-nonadecene-11-one in two sex pheromones, whereas only PBP3 exhibited specific affinity towards both these two sex pheromone components, and PBP1 showed a high binding affinity to the sex pheromone components, and to other plant volatiles. In addition, GOBP1–2 displayed high binding affinity to general components of plant volatiles.
CONCLUSION
Our study suggested CsasPBPs and CsasGOBPs play distinct physiological roles in the perception of sex pheromones and host plant volatiles.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Gui-Sheng Qiu

Research topic(s) for pests/diseases/weeds:
pheromones/attractants/traps

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Carposina sasakii