Pest Management Science (2019) 75, 2271-2277

Sachiyo Sanada-Morimura, Tomohisa Fujii, Ho Van Chien, Le Quoc Cuong, Gerardo F. Estoy Jr and Masaya Matsumura (2019)
Selection for imidacloprid resistance and mode of inheritance in the brown planthopper, Nilaparvata lugens
Pest Management Science 75 (8), 2271-2277
Abstract:
BACKGROUND
Strong resistance to imidacloprid in Nilaparvata lugens (Stål) has developed in Southeast and East Asia. Although the mode of inheritance for resistance is very useful information for pest control, this information is unknown in N. lugens. Here, we established two resistant strains that were selected from field populations in Vietnam and the Philippines, and conducted crossing experiments to determine the inheritance pattern.
RESULTS
The resistance ratio of 50% lethal dose (LD₅₀) values for the two resistance-selected strains, i.e., resistant strains originating from Vietnam (VT-Res) and the Philippines (PH-Res), to their control strains were ~ 8- and 157-fold, respectively. Reciprocal cross experiments between VT-Res and the susceptible strain (S-strain), and between PH-Res and the S-strain showed that the degree of dominance was 0.81 and 0.82, and 0.95 and 0.96, respectively. Analysis of the F₂ populations and backcrosses to the parental strains indicated that resistance is a major-gene trait following Mendelian inheritance. The strength of the resistance was suppressed by pre-treatment with piperonyl butoxide, an inhibitor of cytochrome P450-monooxygenases.
CONCLUSION
Our results suggest that imidacloprid resistance in N. lugens is autosomal and an almost completely dominant major-gene trait that is likely manifested by high expression levels of a detoxification enzyme.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Masaya Matsumura

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Nilaparvata lugens		Philippines
	Nilaparvata lugens		Vietnam