Pest Management Science (2016) 72, 671-683

Costas Ch. Voudouris, Amalia N. Kati, Eldem Sadikoglou, Martin Williamson, Panagiotis J. Skouras, Ourania Dimotsiou, Stella Georgiou, Brian Fenton, George Skavdis and John T. Margaritopoulos (2016)
Insecticide resistance status of Myzus persicae in Greece: long-term surveys and new diagnostics for resistance mechanisms
Pest Management Science 72 (4), 671-683
Abstract:
BACKGROUND
Myzus persicae nicotianae is an important pest in Greece, controlled mainly by neonicotinoids. Monitoring of the aphid populations for resistance mechanisms is essential for effective control.
RESULTS
Two new RFLP-based diagnostics for the detection of the M918T (super-kdr pyrethroid resistance) and nAChR R81T (neonicotinoid resistance) mutations were applied, along with other established assays, on 131 nicotianae multilocus genotypes (MLGs) collected from tobacco and peach in Greece in 2012–2013. Furthermore, we present resistance data from aphid clones (>500, mainly nicotianae) collected in 2006–2007. About half of the clones tested with a diagnostic dose of imidacloprid were tolerant. The R81T mutation was not found in the 131 MLGs and 152 clones examined. Over half (58.6%) of a subset of 29 clones showed a 9–36-fold overexpression of CYP6CY3. M918T was found at low to moderate frequencies. The kdr and MACE mechanisms and carboxylesterase-based resistance were found at high frequency in all years.
CONCLUSION
The aphid retains costly resistance mechanisms even in the absence of pressure from certain insecticides, which could be attributed to factors related to climate and genetic properties of the populations. The indication of build-up of resistance/tolerance to neonicotinoids, related to CYP6CY3 overexpression, is a matter of concern.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): John T. Margaritopoulos, Panagiotis J. Skouras, Martin S. Williamson, Brian Fenton

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Myzus persicae	Peach/nectarine (Prunus persica)	Greece
	Myzus persicae	Tobacco (Nicotiana tabacum)	Greece