Pest Management Science (2013) 69, 457-461

Ralf Nauen, John Vontas, Martin Kaussmann and Katharina Wölfel (2013)
Pymetrozine is hydroxylated by CYP6CM1, a cytochrome P450 conferring neonicotinoid resistance in Bemisia tabaci
Pest Management Science 69 (4), 457-461
Abstract:
BACKGROUND
Resistance to neonicotinoid insecticides such as imidacloprid in the cotton whitefly, Bemisia tabaci, is linked to its hydroxylation by constitutively overexpressed CYP6CM1, a cytochrome P450 enzyme. Here, an investigation was conducted to establish whether CYP6CM1 functionally expressed in Sf9 cells also detoxifies pymetrozine, a selective homopteran feeding blocker known to be cross-resistant to neonicotinoids in whiteflies.
RESULTS
Incubation of pymetrozine with functionally expressed Bemisia CYP6CM1 and subsequent LC-MS/MS analysis revealed a rapid formation of two pymetrozine metabolites by hydroxylation of its heterocyclic 1,2,4-triazine ring system. Enzyme kinetics revealed a Km value of 5.9 ± 0.3 µM and a time-dependent depletion of pymetrozine.
CONCLUSION
The known cross-resistance between imidacloprid, other neonicotinoid insecticides and pymetrozine in B. tabaci is most likely conferred by the very same detoxification mechanism, i.e. a monooxygenase-based hydroxylation mechanism linked to the overexpression of CYP6CM1. These insecticide chemistries should not be alternated in whitefly resistance management strategies.
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Link to article at publishers website
Database assignments for author(s): Ralf Nauen, John Vontas

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bemisia tabaci