Pest Management Science (2013) 69, 217-227

Emmanouil Roditakis, Christina Skarmoutsou, Marianna Staurakaki, María del Rosario Martínez-Aguirre, Lidia García-Vidal, Pablo Bielza, Khalid Haddi, Carmelo Rapisarda, Jean-Luc Rison, Andrea Bassi and Luis A. Teixeira (2013)
Determination of baseline susceptibility of European populations of Tuta absoluta (Meyrick) to indoxacarb and chlorantraniliprole using a novel dip bioassay method
Pest Management Science 69 (2), 217-227
Abstract: BACKGROUND: Tuta absoluta (Meyrick) is one of the most serious pests of tomato recently introduced in the Mediterranean region. A novel bioassay method designed for the accurate determination of insecticide toxicity on T. absoluta (IRAC method No. 022) was validated by three different laboratories [Greece (NAGREF), Italy (UC) and Spain (UPCT)] on European populations.
RESULTS: The insecticides indoxacarb and chlorantraniliprole were used as reference products. The IRAC leaf dip method is easy to perform, producing repeatable, homogeneous responses. LC₅₀ values for indoxacarb ranged between 1.8 and 17.9 mg L⁻¹ (NAGREF), 0.93 and 10.8 mg L⁻¹ (UC) and 0.20 and 0.70 mg L⁻¹ (UPCT), resulting in a tenfold, 12-fold and fourfold difference between the least and most susceptible populations at each laboratory respectively. For chlorantraniliprole, LC₅₀ values ranged between 0.10 and 0.56 mg L⁻¹ (NAGREF), 0.23 and 1.34 mg L⁻¹ (UC) and 0.04 and 0.24 mg L⁻¹ (UPCT), resulting in a sixfold difference in all three cases. Overall, UPCT reported lower mean LC₅₀ to indoxacarb, while UC reported higher LC₅₀ to chlorantraniliprole.
CONCLUSIONS: The new bioassay is reliable, providing a useful tool in the design of IRM strategies. Within each country/lab, the variability observed in the results for both indoxacarb and chlorantraniliprole can be attributed to natural variation. Future research is necessary to determine the extent to which it is possible to compare results among laboratories.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Emmanouil Roditakis

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phthorimaea absoluta		Greece
	Phthorimaea absoluta		Italy
	Phthorimaea absoluta		Spain (continental)