Pest Management Science (2010) 66, 482-489

Sheng-Ming Liu, Yu Chen, Jun-Jie Yu, Chang-Jun Chen, Jian-Xin Wang and Ming-Guo Zhou (2010)
Transfer of the ß-tubulin gene of Botrytis cinerea with resistance to carbendazim into Fusarium graminearum
Pest Management Science 66 (5), 482-489
Abstract: BACKGROUND: Resistance to carbendazim and other benzimidazole fungicides in Botrytis cinerea (Pers. ex Fr.) and most other fungi is usually conferred by mutation(s) in a single chromosomal ß-tubulin gene, often with several allelic mutations. In Fusarium graminearum Schwade, however, carbendazim resistance is not associated with a mutation in the corresponding ß-tubulin gene.
RESULTS: The ß-tubulin gene conferring carbendazim resistance in B. cinerea was cloned and connected with two homologous arms of the ß-tubulin gene of F. graminearum by using a double-joint polymerase chain reaction (PCR). This fragment was transferred into F. graminearum via homologous double crossover at the site where the ß-tubulin gene of F. graminearum is normally located (the ß-tubulin gene of F. graminearum had been deleted). The transformants were confirmed and tested for their sensitivity to carbendazim.
CONCLUSION: The ß-tubulin gene conferring carbendazim resistance in B. cinerea could not express this resistance in F. graminearum, as transformants were still very sensitive to carbendazim.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Ming-Guo Zhou

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Botrytis cinerea
	Fusarium graminearum