Pest Management Science (2000) 56, 448-454

Bing-Lan Liu, Yew-Min Tzeng and Chao-Te Wei (2000)
Recovery of solubilized delta-endotoxin from Bacillus thuringiensis subsp kurstaki fermentation broth
Pest Management Science 56 (5), 448-454
Abstract: Insecticidal delta-endotoxin proteins, degraded from parasporal crystals by protease, were recovered by a simple procedure using heat treatment, solubilization, and ultrafiltration of a fermentation broth of Bacillus thuringiensis subsp kurstaki HD-1. A 68 kDa insecticidal protein was obtained and characterized by SDS-PAGE. The procedure described gave a nearly quantitative recovery of toxicity. Furthermore, bioassay results on larvae of the diamondback moth (Plutella xylostella) showed that the 68 kDa delta-endotoxin fraction (P1) was the principal insecticidal component to this target insect. A similar molecular mass polypeptide P2 (65 kDa) which was solubilized together with P1 from the parasporal crystals, gave relatively low mortality.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Yew-Min Tzeng

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
rearing/culturing/mass production

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plutella xylostella
Bacillus thuringiensis var. kurstaki (entomopathogen)	Plutella xylostella