Pesquisa Agropecuária Brasileira (2000) 35, 719-725

A.K. Kobayashi and L.G.E. Vieira (2000)
Establishment of an in vitro system for studies on the induced resistance of cotton to Xanthomonas campestris pv. malvacearum
Pesquisa Agropecuária Brasileira 35 (4), 719-725
Abstract: An in vitro system for studying the resistance response of cotton (Gossypium hirsutum L.) to Xanthomonas campestris pv. malvacearum was investigated. Cell suspension cultures, established from hypocotyl-derived callus of cotton cultivar 101-102B, were treated with bacterial extracellular polysaccharides (EPS) extracted from the incompatible race 18 of X. campestris pv. malvacearum. EPS at 600 mg/mL caused pronounced darkening of the suspension cultures, as indicative of cell death, 48 hours after incubation. Protein electrophoresis analysis of the time course of EPS-treated cells showed differential accumulation of several protein bands after 12-24 hours. The time course of protein accumulation and cell death was consistent with an elicitor-mediated hypersensitive response.
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Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas citri subsp. malvacearum	Cotton (Gossypium)