Pathogens (2021) 10 (5 - 574)

Evanthia Xylogianni, Paolo Margaria, Dennis Knierim, Kyriaki Sareli, Stephan Winter and Elisavet K. Chatzivassiliou (2021)
Virus surveys in olive orchards in Greece identify Olive virus T, a novel member of the genus Tepovirus
Pathogens 10 (5 - 574)
Abstract: Field surveys were conducted in Greek olive orchards from 2017 to 2020 to collect information on the sanitary status of the trees. Using a high-throughput sequencing approach, viral sequences were identified in total RNA extracts from several trees and assembled to reconstruct the complete genomes of two isolates of a new viral species of the genus Tepovirus (Betaflexiviridae), for which the name olive virus T (OlVT) is proposed. A reverse transcription–polymerase chain reaction assay was developed which detected OlVT in samples collected in olive growing regions in Central and Northern Greece, showing a virus prevalence of 4.4% in the olive trees screened. Sequences of amplified fragments from the movement–coat protein region of OlVT isolates varied from 75.64% to 99.35%. Three olive varieties (Koroneiki, Arbequina and Frantoio) were infected with OlVT via grafting to confirm a graft-transmissible agent, but virus infections remained latent. In addition, cucumber mosaic virus, olive leaf yellowing-associated virus and cherry leaf roll virus were identified.
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Database assignments for author(s): Paolo Margaria, Stephan Winter, Elisavet K. Chatzivassiliou

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Olive virus T	Olive (Olea europaea)	Greece