New Phytologist (2012) 196, 873-886

Gaëlle Pagny, Pauline S. Paulstephenraj, Sylvain Poque, Ophélie Sicard, Patrick Cosson, Jean-Philippe Eyquard, Mélodie Caballero, Aurélie Chague, Germain Gourdon, Lise Negrel, Thierry Candresse, Stéphanie Mariette and Véronique Decroocq (2012)
Family-based linkage and association mapping reveals novel genes affecting Plum pox virus infection in Arabidopsis thaliana
New Phytologist 196 (3), 873-886
Abstract: Sharka is a devastating viral disease caused by the Plum pox virus (PPV) in stone fruit trees and few sources of resistance are known in its natural hosts. Since any knowledge gained from Arabidopsis on plant virus susceptibility factors is likely to be transferable to crop species, Arabidopsis's natural variation was searched for host factors essential for PPV infection.
To locate regions of the genome associated with susceptibility to PPV, linkage analysis was performed on six biparental populations as well as on multiparental lines. To refine quantitative trait locus (QTL) mapping, a genome-wide association analysis was carried out using 147 Arabidopsis accessions.
Evidence was found for linkage on chromosomes 1, 3 and 5 with restriction of PPV long-distance movement. The most relevant signals occurred within a region at the bottom of chromosome 3, which comprises seven RTM3-like TRAF domain-containing genes. Since the resistance mechanism analyzed here is recessive and the rtm3 knockout mutant is susceptible to PPV infection, it suggests that other gene(s) present in the small identified region encompassing RTM3 are necessary for PPV long-distance movement.
In consequence, we report here the occurrence of host factor(s) that are indispensable for virus long-distance movement.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Véronique Decroocq

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plum pox virus