Neotropical Entomology (2018) 47, 145-151

L.P. Ribeiro, O.Z. Zanardi, G.L.P. Gonçalves, T.F. Ansante, P.T. Yamamoto and J.D. Vendramim (2018)
Toxicity of an annonin-based commercial bioinsecticide against three primary pest species of stored products
Neotropical Entomology 47 (1), 145-151
Abstract: The effects of a bioinsecticide formulation based on extract of Annona squamosa L. (Annonaceae) containing 10,000 mg L^-1 of acetogenin annonin as the main active ingredient were investigated against three primary pest species of stored grains in Brazil [maize weevil Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), Mexican bean weevil Zabrotes subfasciatus (Boheman) (Coleoptera: Chrysomelidae: Bruchinae), and cowpea weevil Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae: Bruchinae)] by means of residual contact bioassays. In a concentration-dependent manner, the annonin-based commercial bioinsecticide caused significant adult mortality of C. maculatus (LC₅₀ = 6890 μL kg^-1), S. zeamais (LC₅₀ = 2781 μL kg^-1), and Z. subfasciatus (LC₅₀ = 2120 μL kg^-1) after 120 h of residual contact exposure. In addition to acute toxicity, the tested bioinsecticide also promoted a significant reduction of the number of eggs laid by females of C. maculatus (EC₅₀ = 5949.7 μL kg^-1) and Z. subfasciatus (EC₅₀ = 552.7 μL kg^-1). Moreover, the bioinsecticide significantly reduced the number of emerged insects (F₁ generation) of C. maculatus (EC₅₀ = 2763.0 μL kg^-1), S. zeamais (EC₅₀ = 1380.8 μL kg^-1), and Z. subfasciatus (EC₅₀ = 561.5 μL kg^-1). The bioinsecticide also reduced the percentage of damaged grains for the three pest species studied, and its grain-protectant properties are comparable to or superior in efficacy in relation to a diatomaceous earth-based insecticide (Insecto® at 1000 mg kg^-1) used as a positive control. Thus, this standardized formulation has promising bioactivity against stored insect species and can be a useful component for IPM of stored grains in Brazil and elsewhere.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Odimar Zanuzo Zanardi, José Djair Vendramim, Pedro T. Yamamoto

Research topic(s) for pests/diseases/weeds:
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Zabrotes subfasciatus
	Callosobruchus maculatus
	Sitophilus zeamais