Nematropica (2012) 42, 96-107

E. Fernández-Herrera, R.I. Rojas-Martínez, L. Guevara-Olvera, M.E. Rivas-Dávila, E. Valadez-Moctezuma and E. Zavaleta-Mejía (2012)
Defensa en chile cm-334 inoculado con Phytophthora capsici e infecta do por Nacobbus aberrans
[Defense in chili cm-334 inoculated with Phytophthora capsici and infected by Nacobbus aberrans]
Nematropica 42 (1), 96-107
Abstract: The transcript accumulation of the genes POX (peroxidase), GLU (β-1,3-glucanase), PR-1 (pathogenesis-related protein 1) and EAS (5-epi-aristolochene synthase, involved in capsidiol biosynthesis), the activity of peroxidase and glucanase, and the content of capsidiol in roots of chili (Capsicum annuum) CM-334 inoculated with Nacobbus aberrans (Na), Phytophthora capsici (Pc) and both pathogens were determined. Plants of CM-334 were inoculated with 2,000 J2 of N. aberrans and/or 300, 000 zoospores of P. capsici. At 6, 12, 24 and 48 h after of inoculation (hai) with the oomycete the expression of the mentioned genes was analyzed by real time PCR, and the activity of β-1,3-glucanase and peroxidase and the content of capsidiol were determined. In chili CM-334 inoculated only with Pc the EAS transcripts showed the maximum expression (23.4 times) at 6 hai, whereas in the interaction Na-Pc, there was a significant reduction (6.9 times) of EAS transcripts at this time. CM-334 plants inoculated with N. aberrans showed low levels of EAS and PRs genes, which contrasted significantly with gene transcript accumulation in the interaction CM-334-P. capsici. The transcripts of PRs were detected from 6 hai, and increased gradually until 48 hai. The activity of β-1,3-glucanase and peroxidase in CM-334 inoculated only with N. aberrans was low with respect to those inoculated with P. capsici or both pathogens, where the activity was significantly greater (P ≤ 0.05) at 12, 24 and 48 hai. In roots of CM-334 plants inoculated only with nematode the content of capsidiol was reduced by 31.6, 54.4, 57.2 and 66.6% with respect to the inoculated only with P. capsici; however in the roots where P. capsici was present (treatments Pc and Na-Pc), significant (P ≤ 0.05) greater levels were registered.
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(original language: Spanish)
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Database assignments for author(s): Emma Zavaleta-Mejia

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytophthora capsici	Green pepper/chilli (Capsicum)
	Nacobbus aberrans	Green pepper/chilli (Capsicum)