Nematropica (2009) 39, 311-317

R. Martínez-Fuentes, A. Tovar-Soto, R. Torres-Coronel and A.E. Brechú-Franco (2009)
Histopatología de cultivares de fríjol (Phaseolus vulgaris L.) no hospedantes de Nacobbus aberrans (Thorne, 1935) Thorne y Allen, 1944
[Histopathology of bean (Phaseolus vulgaris L.) cultivars non-host to Nacobbus aberrans (Thorne, 1935) Thorne and Allen]
Nematropica 39 (2), 311-317
Abstract: We describe the histopathological changes caused by a Nacobbus aberrans population from Chapingo, Mexico on bean cultivars Negro Querétaro and Canario, which are non-hosts to this population. The plants were inoculated with 15,000 eggs and evaluated 15, 45, and 90 days after inoculation (dai). For each date, the roots were embedded in Tissue Teck® O.C.T. Compound^TM and 10 µm-thick sections were cut with a Microm HM505N® cryostat at -30°C and then stained with basic fuschsine-fast green. At 15 dai on both cultivars, nematodes were trapped in lignified cavities surrounded by hypertrophied cells. The nematodes present were third (J3) and fourth stage juveniles (J4), identified by morphological characters, and remained in these stages until 90 dai. Genetic variability of the nematode populations and of the host cultivars is discussed as related to the diverse histopathological reaction responses on bean.
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(original language: Spanish)
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Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Nacobbus aberrans	Beans (Phaseolus)	Mexico