Nematology (2018) 20, 271-283

Adrienne M. Gorny, Frank S. Hay, Xiaohong Wang and Sarah J. Pethybridge (2018)
Isolation of nematode DNA from 100 g of soil using Fe₃O₄ super paramagnetic nanoparticles
Nematology 20 (3), 271-283
Abstract: An economical method for extracting nematode DNA from 100 g of soil was developed to facilitate nematode detection and quantification, and tested using the Northern root-knot nematode, Meloidogyne hapla. The method utilised enzymatic laundry detergent lysis, Fe₃O₄ super paramagnetic iron oxide nanoparticle (SPION) capture, and polyvinylpolypyrrolidone (PVPP) purification. Resultant DNA from this SPION capture method was approximately 100-fold less but of similar quality to DNA obtained from a standard phenol procedure and a commercial DNA extraction kit. An addition of 10 mg of nanoparticles to the extraction lysate was identified to maximise DNA yield while minimising co-capture of contaminants. The detection limit of the SPION capture method was approximately 100 nematodes (100 g soil)^-1. The SPION capture method extracted nematode DNA from mineral soils but requires further optimisation for extraction from high organic matter (i.e., 'muck') soils. The benefits of this method compared to alternative techniques are discussed.
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Link to article at publishers website
Database assignments for author(s): Sarah J. Pethybridge, Xiaohong Wang

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Meloidogyne hapla