Nematology (2012) 14, 723-739

Juan Ma, Shulong Chen, Patrick De Clercq, Lieven Waeyenberge, Richou Han and Maurice Moens (2012)
A new entomopathogenic nematode, Steinernema xinbinense n. sp. (Nematoda: Steinernematidae), from north China
Nematology 14 (6), 723-739
Abstract: During a survey for entomopathogenic nematodes in northern China, a new species of Steinernema was isolated from soil samples collected from Xinbin county, Liaoning province. This nematode was obtained by the insect-baiting technique using last instar larvae of Galleria mellonella. It is described herein as S. xinbinense n. sp. The nematode can be separated from other described species of the group by morphological and morphometric characteristics of the different stages and by characterisation and phylogeny of DNA sequences of the D2D3 domain of the LSU or ITS regions of rDNA. This new species is characterised by the following morphological characters: infective third-stage juvenile with a body length of 694 (635-744) μm, distance from head to excretory pore of 51 (46-53) μm, tail length of 73 (61-81) μm, E = 71 (65-78)%, presence of eight unevenly spaced and developed ridges in middle lateral field (i.e., nine lines). First generation male with well curved, yellowish spicules 56 (49-62) μm long and gubernaculum 35 (30-41) μm long, small mucron mostly present, first generation female with protruding vulva, tail conical with one or two small mucrons and D = 45 (41-50)%. Cross hybridisation tests with S. tielingense, S. kraussei, S. feltiae and S. hebeiense showed that this species was reproductively isolated. The analyses of ITS-rDNA and D2D3 sequence confirm that the studied nematode isolate is a valid new species belonging to the 'feltiae-kraussei-oregonense' group.
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Link to article at publishers website
Database assignments for author(s): Patrick De Clercq, Lieven Waeyenberge

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Steinernema xinbinense (entomopathogen)			China (NE)