Nematology (2011) 13, 589-605

Hari Bahadur Khatri-Chhetri, Lieven Waeyenberge, Sergei Spiridonov, Hira Kaji Manandhar and Maurice Moens (2011)
Steinernema lamjungense n. sp. (Rhabditida: Steinernematidae), a new species of entomopathogenic nematode from Lamjung district, Nepal
Nematology 13 (5), 589-605
Abstract: A new species of entomopathogenic nematode, Steinernema lamjungense n. sp., was recovered from soil samples collected from Tarku, Lamjung district of Nepal, during a survey in 2007. The analysis of ITS-rDNA sequence placed S. lamjungense n. sp. in the arenarium-glaseri-karii-longicaudum group. The nematode can be separated from other described species of the group by morphological and morphometric characteristics of different stages and by characterisations and phylogeny of DNA sequences of the D2D3 domain of the LSU or ITS regions of rDNA. For infective juveniles, the new species can be recognised by medium-long body length of 832 (690-950) μm, pharynx length of 127 (110-142) μm, H% = 61 (55-69) and E% = 79 (69-97). The lateral field pattern is 2, 6, 7, 8, 3, 2. The males have well curved, yellowish-brown spicules with a prominent velum. The spicule terminus is blunt with a longitudinal ventral notch. Spicule length is 87 (81-94) μm and gubernaculum length is 57 (50-66) μm in the first generation males. A tail mucron is absent in first but present in second generation males. First generation males have a lateral field with a single ridge. A post-anal swelling is not developed in first generation females but is well developed in second generation females. Three other isolates of the same species were recovered from the neighbourhood of the type locality and yet another isolate was collected from Swarketari, Syangja district.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Lieven Waeyenberge, Sergei E. Spiridonov

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Steinernema lamjungense (entomopathogen)			Nepal