Nematology (2000) 2, p. 780 (Papert et al.)

Artemis Papert and C.J. (Hans) Kok (2000)
DNA fingerprinting of bacterial communities of Meloidogyne fallax egg masses
Nematology 2 (7), 780-780
Presented at the 25th International Nematology Symposium, Herzliya, Israel, 2-7 April 2000
Abstract: The gelatinous matrix in which Meloidogyne eggs are embedded is considered to be a protection against physical stress on the eggs and against egg parasitic microorganisms. The bacterial community of the gelatinous matrix may influence the protective action of the matrix, by being antagonistic either to the nematode eggs or to egg parasitic micro-organisms. The bacterial community of Meloidogyne fallax egg masses was analysed with Denaturing Gradient Gel Electrophoresis (DGGE) of PCR-amplified 16S DNA fragments. This technique yields a DNA-fingerprint of the major bacterial species in the community, including the non-culturable species. Samples were taken from egg masses and rhizoplane of potato, growing in pots with field soil from two experimental fields in the Netherlands. The DNA fingerprints show that the bacterial community of egg masses is different from the bacterial community of the rhizoplane. Furthermore, site-specific differences in the communities were found. Our conclusion is that the species composition of the bacterial community of the egg masses of Meloidogyne fallax is different from the composition of adjacent rhizoplane communities. This stresses the potential importance of the bacterial egg mass community as an ecological factor in the reproduction efficiency of the nematode and the biological control of Meloidogyne.
Database assignments for author(s): C. Johannes Kok

Research topic(s) for pests/diseases/weeds:
environment - cropping system/rotation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Meloidogyne fallax		Netherlands