Nematology (2000) 2, p. 772 (Liu et al.)

Jia Liu, Hinanit Koltai, Nor Chejanovsky and Yitzhak Spiegel (2000)
Protein MJE36: a stage- and genus-specific putative collagen gene product detected in Meloidogyne javanica
Nematology 2 (7), 772-772
Presented at the 25th International Nematology Symposium, Herzliya, Israel, 2-7 April 2000
Abstract: We have cloned and expressed a fusion protein containing a nonconserved 58-amino acid sequence encoded by a DNA sequence between the homology block A and the first (Gly-X-Y)n block from Meloidogyne javanica Mjcol-4 gene. Rabbit antiserum, which was raised against the fusion protein, detected a strong reacting protein band (Mw 36 kDa) on western blots of M. javanica eggs extracted by 2-mercaptoethanol (BME). This protein was designated as MJE36 and was found to be collagenase sensitive. MJE36 was not detected on western blots of M. javanica second-stage juveniles (J2) or adult females extracted by BME, which suggests that MJE36 expression is stage-specific. A band of the same molecular size was detected with M. incognita eggs extracted by BME but not detected with Heterodera avenae. These findings imply that the expression is also genus-specific. M. javanica eggs immunolabelling assays showed that the antiserum reacted only with the broken M. javanica eggs densely and irregularly, while no reacting bands were detected with purified M. javanica eggshells extracted by BME, suggesting that MJE36 was not in M. javanica eggshells.
Database assignments for author(s): Nor Chejanovsky

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Meloidogyne javanica