Mycologia (2002) 94, 404-410

Mohamed Tribak, Juan A. Ocampo and Inmaculada García-Romera (2002)
Production of xyloglucanolytic enzymes by Trichoderma viride, Paecilomyces farinosus, Wardomyces inflatus, and Pleurotus ostreatus
Mycologia 94 (3), 404-410
Abstract: Different conditions of culture medium, incubation time, concentration and surfactant were tested to determine xyloglucanase activity. Trichoderma viride, Paecilomyces farinosus, Wardomyces inflatus and Pleurotus ostreatus showed increased xyloglucanase activities when the fungi grown on microcrystalline cellulose as the sole carbon source. Endoxyloglucanase activity increased with the growth of the fungi and reached a peak on day 14 of incubation, practically 95% of the activity was associated with the extracellular fraction. Precipitation with ammonium sulfate was the best concentration method for detection of endoxyloglucanase activity of the fungi. Endoxyloglucanase activity of the fungi was increased by 4 fold with the use of the non-ionic surfactant Tween 20. Six and three bands of xyloglucanase activities were observed in T. viride and P. ostreatus, respectively, whereas both P. farinosus and W. inflatus presented only one xyloglucanase activity band. These results indicate the presence of several xyloglucanases in the saprophytic fungi examined.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Cordyceps farinosa (entomopathogen)
Trichoderma viride (antagonist)