Molecular Plant Pathology (2009) 10, 777-793

Kathy R. Munkvold and Gregory B. Martin (2009)
Advances in experimental methods for the elucidation of Pseudomonas syringae effector function with a focus on AvrPtoB
Molecular Plant Pathology 10 (6), 777-793
Abstract: Pseudomonas syringae infects a wide range of plant species through the use of a type III secretion system. The effector proteins injected into the plant cell through this molecular syringe serve as promoters of disease by subverting the plant immune response to the benefit of the bacteria in the intercellular space. The targets and activities of a subset of effectors have been elucidated recently. In this article, we focus on the experimental approaches that have proved most successful in probing the molecular basis of effectors, ranging from loss-of-function to gain-of-function analyses utilizing several techniques for effector delivery into plants. In particular, we highlight how these diverse approaches have been applied to the study of one effector-AvrPtoB-a multifunctional protein with the ability to suppress both effector-triggered immunity and pathogen (or microbe)-associated molecular pattern-triggered immunity. Taken together, advances in this field illustrate the need for multiple experimental approaches when elucidating the function of a single effector.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pseudomonas syringae