Molecular Plant Pathology (2000) 1, 195-199

Dawn Louise Arnold, Robert Wilson Jackson and Alan Vivian (2000)
Evidence for the mobility of an avirulence gene, avrPpiA1, between the chromosome and plasmids of races of Pseudomonas syringae pv. pisi
Molecular Plant Pathology 1 (3), 195-199
Abstract: An 8.5-kb DNA fragment containing the avirulence gene avrPpiA1 is present in the chromosome of race 2 but not present in race 4B of Pseudomonas syringae pv. pisi. The likely acquisition of avrPpiA1 in race 2 at some time in the past was indicated by the presence of direct repeat sequences at the boundaries of the 8.5-kb region, which was amplified by polymerase chain reaction (PCR), cloned and sequenced. The sequence analysis of the 8.5-kb fragment revealed the presence of rulAB genes, which are usually associated with plasmids. The rulB gene had been disrupted by a 4.3-kb length of DNA, which included avrPpiA1 and open reading frames (ORFs) with similarity to bacteriophage and transposase genes. Inverted repeats flanked the 4.3-kb region reinforcing the possibility that a further insertion had occurred in this region. The results presented here provide evidence for the horizontal transfer of an avirulence gene and suggest that this region may be a hotspot for recombination with the chromosome.
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Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pseudomonas syringae pv. pisi
	Pseudomonas syringae