Molecular Plant-Microbe Interactions (2012) 25, 231-240

Gal Nissan, Shulamit Manulis-Sasson, Laura Chalupowicz, Doron Teper, Adva Yeheskel, Metsada Pasmanik-Chor, Guido Sessa and Isaac Barash (2012)
The type III effector HsvG of the gall-forming Pantoea agglomerans mediates expression of the host gene HSVGT
Molecular Plant-Microbe Interactions 25 (2), 231-240
Abstract: The type III effector HsvG of the gall-forming Pantoea agglomerans pv. gypsophilae is a DNA-binding protein that is imported to the host nucleus and involved in host specificity. The DNA-binding region of HsvG was delineated to 266 amino acids located within a secondary structure region near the N-terminus of the protein but did not display any homology to canonical DNA-binding motifs. A binding site selection procedure was used to isolate a target gene of HsvG, named HSVGT, in Gypsophila paniculata. HSVGT is a predicted acidic protein of the DnaJ family with 244 amino acids. It harbors characteristic conserved motifs of a eukaryotic transcription factor, including a bipartite nuclear localization signal, zinc finger, and leucine zipper DNA-binding motifs. Quantitative real-time polymerase chain reaction analysis demonstrated that HSVGT transcription is specifically induced in planta within 2 h after inoculation with the wild-type P. agglomerans pv. gypsophilae compared with the hsvG mutant. Induction of HSVGT reached a peak of sixfold at 4 h after inoculation and progressively declined thereafter. Gel-shift assay demonstrated that HsvG binds to the HSVGT promoter, indicating that HSVGT is a direct target of HsvG. Our results support the hypothesis that HsvG functions as a transcription factor in gypsophila.
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Link to article at publishers website
Database assignments for author(s): Shulamit Manulis-Sasson, Guido Sessa

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pantoea agglomerans	Gypsophila (crop)