Molecular Plant-Microbe Interactions (2009) 22, 1143-1150

Yan-Jun Zhang, Jun-Jie Yu, Yan-Nan Zhang, Xiao Zhang, Chang-Jun Cheng, Jan-Xin Wang, Derek W. Hollomon, Ping-Sheng Fan and Ming-Guo Zhou (2009)
Effect of carbendazim resistance on trichothecene production and aggressiveness of Fusarium graminearum
Molecular Plant-Microbe Interactions 22 (9), 1143-1150
Abstract: Fusarium graminearum (teleomorph, Gibberella zeae) causes head blight of cereals and contaminates grains with trichothecene mycotoxins that are harmful to humans and domesticated animals. Control of Fusarium head blight relies on carbendazim (MBC) in China, but resistance to MBC in F. graminearum is now widespread. Sixty-seven strains were evaluated for trichothecene production in shake culture or in the field. The strains included 60 wild-type strains (30 MBC-resistant and 30 MBC-sensitive), three MBC-resistant site-directed mutants at codon 167 in ß₂-tubulin, three MBC-sensitive site-directed mutants at codon 240 in ß₂-tubulin, and their MBC-sensitive wild-type progenitor strain ZF21. The incidence of infected spikelets and the amount of F. graminearum DNA in field grain (AFgDNA) also were evaluated for all strains. MBC resistance increased trichothecene production in shake culture or in the field. Although MBC resistance did not change the incidence of infected spikelets, it did increase AFgDNA. Tri5 gene expression increased in MBC-resistant strains grown in shake culture. We found a significant exponential relationship between trichothecene production and Tri5 gene expression in shake culture and a linear relationship between the incidence of infected spikelets or AFgDNA and trichothecene production in field grain.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Ming-Guo Zhou, Derek W. Hollomon

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium graminearum