Molecular Plant-Microbe Interactions (1999) 12, 845-851

Sylwia Jafra, Izabela Figura, Nicole Hugouvieux-Cotte-Pattat and Ewa Lojkowska (1999)
Expression of Erwinia chrysanthemi pectinase genes pelI, pelL, and pelZ during infection of potato tubers
Molecular Plant-Microbe Interactions 12 (10), 845-851
Abstract: Erwinia chrysanthemi mutants, containing transcriptional fusions of one of the minor pectate lyase genes (pelI, pelL, pelZ) with the reporter gene encoding beta-glucuronidase activity, were studied for their ability to cause disease symptoms and to synthesize pectinases after inoculation of potato tubers. The strains affected in pelI and pelL genes displayed reduced virulence on potato tubers, demonstrating the important role of these isoenzymes in soft rot disease. Inactivation of the pelZ gene slightly influences the ability to macerate. Analysis of the bacterial population showed rapid multiplication of bacteria during infection. Similar kinetics of growth were observed for all mutants and for the wild-type strain. Comparison of the mutants and the wild-type strain showed that the pelI, pelL, and pelZ mutants synthesized reduced levels of Pels. The expression of pelZ is fivefold higher in planta than in bacterial cultures. In contrast, both pelI and pelL are highly (10-fold factor) induced in planta, which is characteristic of the plant-inducible pectate lyases.
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Link to article at publishers website
Database assignments for author(s): Ewa Lojkowska, Sylwia Jafra

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Dickeya (genus)