Molecular Ecology Resources (2008) 8, 1004-1007

N. Gauthier, C. Dalleau-Clouet and M.-E. Bouvret (2008)
Twelve new polymorphic microsatellite loci and PCR multiplexing in the whitefly, Bemisia tabaci
Molecular Ecology Resources 8 (5), 1004-1007
Abstract: Twelve new dinucleotide microsatellite loci of the whitefly, Bemisia tabaci, were obtained from enriched genomic libraries. Three polymerase chain reaction multiplex sets comprising three, five and four loci were optimized and characterized across 133 B. tabaci females from Israeli rearings and natural populations collected in four Mediterranean countries (Tunisia, France, Spain and Morocco). There were three to 24 alleles per locus and the observed heterozygosity was from 0.084 to 0.420. Deviation from Hardy-Weinberg equilibrium was detected at four loci associated with significant heterozygote deficiencies due to null alleles and presence of subpopulations that were mostly in the Tunisian sample. The 12 loci carried independent information.
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Link to article at publishers website
Database assignments for author(s): Nathalie Gauthier

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bemisia tabaci		France
	Bemisia tabaci		Spain (continental)
	Bemisia tabaci		Morocco
	Bemisia tabaci		Tunisia
	Bemisia tabaci		Israel