Molecular Ecology Notes (2006) 6, 543-546

Felicity J. Keiper, Matthew J. Hayden and Hugh Wallwork (2006)
Development of sequence tagged microsatellites (STMs) for the barley scald pathogen Rhynchosporium secalis
Molecular Ecology Notes 6 (2), 543-546
Abstract: A rapid and cost efficient technique was developed and used to generate 168 sequence tagged microsatellites (STMs) in the barley scald pathogen Rhynchosporium secalis. Sixty-two STMs, amplifying 66 loci, revealed a high level of polymorphism among a diverse set of 16 Australian isolates. Each locus revealed two to nine alleles (average 4 ± 1.82), and a gene diversity measure of 0.54 was obtained. This technique not only halved the cost of marker development compared to traditional methods, but substantially reduced the cost of performing fluorescence-based microsatellite assays. These STMs provide a powerful tool for genetic studies in R. secalis.
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Link to article at publishers website
Database assignments for author(s): Hugh Wallwork

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Rhynchosporium secalis