Molecular Ecology Notes (2005) 5, 558-561

G. Nakabonge, M.N. Cortinas, J. Roux, M. Gryzenhout, B.D. Wingfield and M.J. Wingfield (2005)
Development of polymorphic microsatellite markers for the fungal tree pathogen Cryphonectria eucalypti
Molecular Ecology Notes 5 (3), 558-561
Abstract: Polymorphic microsatellite DNA markers were developed from a single spore isolate of Cryphonectria eucalypti collected from a Eucalyptus stem canker in South Africa. Markers were obtained using the enrichment technique known as fast isolation by AFLPs of sequences containing repeats (FIASCO). Ten polymorphic markers were isolated, of which, two were discarded due to their high polymorphism in the flanking region. The mean number of alleles produced by the remaining eight markers from 20 isolates was 7.25, and alleles per locus ranged from four to 12. The markers will be used to study populations of C. eucalypti.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Brenda D. Wingfield, Michael J. Wingfield, Jolanda Roux, Marieka Gryzenhout

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Holocryphia eucalypti	Eucalypt (Eucalyptus)	South Africa