Molecular Ecology Notes (2005) 5, 546-548

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Celeste C. Linde, Marcello Zala and Bruce A. McDonald (2005)
Isolation and characterization of microsatellite loci from the barley scald pathogen, Rhynchosporium secalis
Molecular Ecology Notes 5 (3), 546-548
Abstract: Fifteen primer pairs were designed for 14 polymorphic microsatellite loci, which were isolated and characterized from genomic libraries of Rhynchosporium secalis. Conditions for multiplexing and simultaneous genotyping of up to eight loci in a single run are described. The number of alleles per polymorphic locus ranged from two to 13 in populations from Switzerland and Australia. Genotypic diversity ranged from 61.5 to 66.7. Gene diversity ranged from 0.08 to 0.89 for individual polymorphic loci, with averages of 0.54 and 0.62 for the populations from Switzerland and Australia, respectively. Variable levels of polymorphism make these ideal markers for population genetic analyses.
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Database assignments for author(s): Celeste C. Linde

Research topic(s) for pests/diseases/weeds:
molecular biology - genes


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Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Rhynchosporium secalis Switzerland