Molecular Ecology Notes (2004) 4, 547-550

Maria M. Geldenhuis, Jolanda Roux, Michael J. Wingfield and Brenda D. Wingfield (2004)
Development of polymorphic markers for the root pathogen Thielaviopsis basicola using ISSR-PCR
Molecular Ecology Notes 4 (4), 547-550
Abstract: Thielaviopsis basicola is a soil-borne fungal pathogen affecting many important agricultural crops. Little is known regarding the population biology or origin of this pathogen. Polymorphic markers developed for Ceratocystis fimbriata, a species complex phylogenetically closely related to T. basicola, were tested and found not to be useful for T. basicola. In this study 14 primer pairs, seven of which resulted in the amplification of single polymorphic fragments in T. basicola were developed. These primers will enable further studies on this economically important pathogen, and will result in an enhanced understanding of its population structure in different parts of the world.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Brenda D. Wingfield, Michael J. Wingfield, Jolanda Roux

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Berkeleyomyces basicola