Molecular Ecology Notes (2003) 3, 425-427

Aditi Pai, Igor V. Sharakhov, Olga Braginets, Christina Costa and Guiyun Yan (2003)
Identification of microsatellite markers in the red flour beetle, Tribolium castaneum
Molecular Ecology Notes 3 (3), 425-427
Abstract: We isolated and characterized microsatellite markers for the red flour beetle Tribolium castaneum, an important model species for studies in various areas of evolutionary biology and ecology. A microsatellite-enriched genomic library was constructed and screened with single stranded oligonucleotide probes [(CCT)₁₇, (AAT)₁₇ and (CAG)₁₇]. Forty-five primer pairs were designed of which 19 pairs produced successful amplification. Polymorphism screening involved beetles from five beetle strains and revealed 15 polymorphic and four monomorphic markers. The development of polymorphic microsatellite markers will facilitate future ecological and genetic studies involving T. castaneum beetles.
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Link to article at publishers website
Database assignments for author(s): Igor V. Sharakhov

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tribolium castaneum