Microbial Ecology (2021) 81, 93-109

Lyza Hery, Amandine Guidez, Audrey-Anne Durand, Christelle Delannay, Josiann Normandeau-Guimond, Yann Reynaud, Jean Issaly, Daniella Goindin, Grégory Legrave, Joel Gustave, Stéphanie Raffestin, Sebastien Breurec, Philippe Constant, Isabelle Dusfour, Claude Guertin and Anubis Vega-Rúa (2021)
Natural variation in physicochemical profiles and bacterial communities associated with Aedes aegypti breeding sites and larvae on Guadeloupe and French Guiana
Microbial Ecology 81 (1), 93-109
Abstract: Aedes aegypti develop in aquatic habitats in which mosquito larvae are exposed to physicochemical elements and microorganisms that may influence their life cycle and their ability to transmit arboviruses. Little is known about the natural bacterial communities associated with A. aegypti or their relation to the biotic and abiotic characteristics of their aquatic habitats. We characterized the physicochemical properties and bacterial microbiota of A. aegypti breeding sites and larvae on Guadeloupe and in French Guiana. In addition, we explored whether geographic location, the type of breeding site and physicochemical parameters influenced the microbiota associated with this mosquito species. We used large-scale 16S rRNA gene sequencing of 160 breeding sites and 147 pools of A. aegypti larvae and recorded 12 physicochemical parameters at the sampled breeding sites. Ordination plots and multiple linear regression were used to assess the influence of environmental factors on the bacterial microbiota of water and larvae. We found territory-specific differences in physicochemical properties (dissolved oxygen, conductivity) and the composition of bacterial communities in A. aegypti breeding sites that influenced the relative abundance of several bacteria genera (e.g., Methylobacterium, Roseoccocus) on the corresponding larvae. A significant fraction of the bacterial communities identified on larvae, dominated by Herbiconiux and Microvirga genera, were consistently enriched in mosquitoes regardless the location. In conclusion, territory-specific differences observed in the biotic and abiotic properties of A. aegypti breeding sites raise concern about the impact of these changes on pathogen transmission by different A. aegypti populations.
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Database assignments for author(s): Isabelle Dusfour, Claude Guertin

Research topic(s) for pests/diseases/weeds:
environment - cropping system/rotation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aedes aegypti		French Guiana
	Aedes aegypti		Guadeloupe