Journal of the American Mosquito Control Association (2003) 19, 367-370

Hall Kasap, Davut Alptekn, Mülkye Kasap, Al. Güzel and Ümt Lüleyap (2003)
Artificial bloodfeeding of Anopheles sacharovi on a membrane apparatus
Journal of the American Mosquito Control Association 19 (4), 367-370
Abstract: Anopheles sacharovi, the main human malaria vector in Turkey, has been maintained in our laboratory by feeding on anesthetized rabbits for about 20 years but it is a difficult species to colonize and bloodfeed. To eliminate the need for keeping and using live rabbits to supply blood meals, artificial bloodfeeding methods with suitable membrane apparatus were investigated. The feeding apparatus designed by the World Health Organization and 3 other types designed by us (for feeding on preserved human blood) were tested. Artificial membranes (latex and paraffin film) and locally produced and dried calf intestine were used. The calf intestine membrane gave the best feeding results and a modified apparatus designated type III was the most successful. This apparatus was preferable for the artificial feeding of An. sacharovi because it has a small reservoir, is easy to use, is adaptable to different feeding conditions, and supports reasonably high bloodfeeding rates 44.4-50.5% as compared to 35% on live rabbits.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)

Research topic(s) for pests/diseases/weeds:
rearing/culturing/mass production

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles sacharovi		Turkey