Journal of the American Mosquito Control Association (1999) 15, 565-568

B.D. Brooke, R.H. Hunt, L.L. Koekemoer, J. Dossou-Yovo and M. Coetzee (1999)
Evaluation of a polymerase chain reaction assay for detection of pyrethroid insecticide resistance in the malaria vector species of the Anopheles gambiae complex
Journal of the American Mosquito Control Association 15 (4), 565-568
Abstract: A published polymerase chain reaction assay proved reliable for detecting nerve-insensitivity (kdr-type) resistant and susceptible alleles to the pyrethroid insecticide permethrin in the malaria vectors Anopheles gambiae s.s. and An. arabiensis and the nonvector species An. quadriannulatus. The assay detects pyrethroid susceptible and resistance alleles mediated by a mutation in region II of the para-type sodium channel gene, showing that resistance is conferred by pyrethroid target site insensitivity. Analysis of results of the assay suggests that more than 1 mechanism conferring pyrethroid resistance is operating in an An. gambiae s.s. strain from Côte d'Ivoire. The value of the assay as a tool for monitoring this mode of insecticide resistance in wild populations is discussed.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Basil D. Brooke, Lizette L. Koekemoer, Maureen Coetzee

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles arabiensis
	Anopheles quadriannulatus