Journal of Vector Ecology (2003) 28, 150-158

Pornpimol Rongnoparut, Soamrutai Boonsuepsakul, Theeraphap Chareonviriyaphap and Naowarat Thanomsing (2003)
Cloning of cytochrome P450, CYP6P5, and CYP6AA2 from Anopheles minimus resistant to deltamethrin
Journal of Vector Ecology 28 (2), 150-158
Abstract: Two new genes in the cytochrome P450 (CYP6) family 6 with complete coding sequences were cloned and sequenced from deltamethrin-resistant Anopheles minimus, a major malaria vector in Thailand. CYP6P5 encodes a protein of 508 amino acids, while CYP6AA2 contains 505 residues. Each encoded protein contains a hydrophobic N-terminal region and a highly conserved heme-binding region typical of P450s. Alignments of deduced amino acid sequences, with other insect P450 genes indicate a high degree of identity to insect CYP6 genes. Comparative mRNA expression studies using semi-quantitative RT-PCR analysis indicated that the relative amount of CYP6AA2 transcript was greater in the deltamethrin-resistant An. minimus compared to the susceptible strain. The expression of CYP6AA2 in deltamethrin-resistant mosquitoes is associated with development of deltamethrin resistance in An. minimus mosquito. The CYP6P5 transcript is equally expressed in both resistant and susceptible mosquitoes.
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Database assignments for author(s): Theeraphap Chareonviriyaphap

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles minimus		Thailand