Journal of Plant Protection Research (2014) 54, 199-203

Fereshteh Vali-Sichani, Masoud Bahar and Leila Zirak (2014)
Characterization of phytoplasmas related to ´Candidatus Phytoplasma asteris' subgroup rpI-L in Iran
Journal of Plant Protection Research 54 (2), 199-203
Abstract: In two of Iran's central provinces, several herbaceous plants showing phytoplasma disease symptoms were collected to detect `Canididatus Phytoplasma asteris'-related phytoplasmas. Confirmation of an association of phytoplasmas with diseased plants was done using polymerase chain reaction (PCR) assays having the phytoplasma universal primer pairs P1/P7 followed by R16F2n/R16R2 in nested PCR. Then, for detection of `Ca. P. asteris', DNA samples were subjected to amplification of rp and tuf genes using specific primer pairs rp(I)F1A/rp(I)R1A and fTufAy/rTufAy, respectively. Restriction fragment length polymorphism or RFLP analyses of rp gene fragments using Tsp509I restriction enzyme as well as sequence analyses indicated that `Ca. P. asteris'-related phytoplasmas associated with carrot, niger seed and scallion plants in these regions, belong to the rpI-L subgroup. This research is the first report of carrot, niger seed, and scallion infection with phytoplasmas belonging to the rpI-L subgroup.
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Database assignments for author(s): Leila Zirak

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytoplasma asteris	Carrot (Daucus carota)	Iran
	Phytoplasma asteris	Onion/garlic/leek (Allium)	Iran
	Phytoplasma asteris	Niger (Guizotia abyssinica)	Iran