Journal of Plant Pathology (2020) 102, 511-515

Marcela Komínková and Petr Komínek (2020)
Development and validation of RT-PCR multiplex detection of grapevine viruses and viroids in the Czech Republic
Journal of Plant Pathology 102 (2), 511-515
Abstract: A multiplex RT-PCR reaction was developed and validated for the simultaneous detection of two viruses and two viroids: grapevine fleck virus, grapevine Pinot gris virus, grapevine yellow speckle viroid 1, and hop stunt viroid. The multiplex reaction specificity was verified on a set of 210 grapevines from the Czech Republic. The results showed the occurrence of hop stunt viroid in 94.3% of the examined plants, while incidence of the other pathogens was lower: grapevine Pinot gris virus (64.8%), grapevine yellow speckle viroid 1 (52.4%), and grapevine fleck virus (15.2%).
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: English)
Link to article at publishers website
Database assignments for author(s): Petr Kominek

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Maculavirus vitis	Grapevine (Vitis)	Czech Republic
	Maculavirus vitis	Grapevine (Vitis)	Czech Republic
	Hop stunt viroid	Grapevine (Vitis)	Czech Republic
	Grapevine yellow speckle viroid 1	Grapevine (Vitis)	Czech Republic