Journal of Plant Pathology (2012) 94, p. S87 (Pappi et al.)

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P.G. Pappi, K.E. Efthimiou and N.I. Katis (2012)
First report of Eggplant mottled dwarf virus in tobacco crops in Albania
Journal of Plant Pathology 94 (4, Suppl.), S87-S87
Abstract: In summer 2008 and 2009, tobacco plants of cvs Lovina and Katerini (Samsun) displaying severe stunting, leaf crinkling and vein clearing (VC) (incidence of diseased plants 0.1%) were observed in areas of Koritsa and Elbasan (Albania). Leaf extract from five affected plants collected in 2009 was used for mechanical inoculations of Nicotiana benthamiana, N. rustica and Gomphrena globosa. Twenty-thirty days post inoculation, N. benthamiana developed a systemic VC, while N. rustica reacted with yellow spots in the inoculated leaves followed by systemic VC. In G. globosa only red local lesions were observed. Leaves from 10 symptomatic and 10 symptomless tobacco plants were collected from different fields and tested serologically by DAS-ELISA using polyclonal antisera to Eggplant mottled dwarf virus (EMDV) (As 0136 antiserum, DSMZ, Braunschweig, Germany). All samples from symptomatic plants tested positive. To confirm the presence of EMDV a one-step RT-PCR as described by Katis et al. (2011) was carried out to the same samples and one of the PCR products was sequenced. BLAST analysis of the obtained sequence revealed 98% nucleotide similarity with the homologous published sequences of the EMDV isolates from tobacco (AM922318), cucumber (AM922317), Pittosporum tobira (AM922320) and 88% with those of virus isolates from eggplant (AM922319) and caper (AM922321), respectively. The obtained sequence was deposited in EMBL-EBI under the accession No. HE601787. EMDV is known since 1969 and is generally considered to be of minor importance. It has been reported to infect tobacco in Italy and Greece (Chatzivassiliou et al., 2004). To our knowledge this is the first report of EMDV in tobacco in Albania, even though its economic importance is limited.
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Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Alphanucleorhabdovirus melongenae Tobacco (Nicotiana tabacum) Albania
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