Journal of Plant Pathology (2007) 89, p. S13 (Battilani et al.)

P. Battilani, V. De Cicco, F. De Curtis, L. Macchia, A. Pietri, A. Ritieni and A. Silva (2007)
Biological and integrated control of Aspergillus carbonarius
Journal of Plant Pathology 89 (3, Suppl.), S13-S13
Oral Presentation at S.I.Pa.V XIV National Meeting - Perugia, Italy 19-21 Sept. 2007
Abstract: The main purpose of this research was to minimize ochratoxin A (OTA) content in grapes. It followed a 'farm to fork' approach and was focussed on protecting consumer's health. Biocontrol agents (BCAs) active against A. carbonarius were studied for their application in organic and integrated farming. The possible effect of BCAs on wine making, the identification of OTA degradation products and their toxicity in mammalian cell models, compared to OTA toxicity, were also studied. During the first year, a biocontrol agent (Aureobasidium pullulans LS30) was applied in two different vineyards, located in Molise and Puglia (southern Italy) both in integrated and organic farming. The BCA became well established in the vineyards, especially in integrated farming, and its population increased over time. No conclusions could be drawn on OTA control because of the very low level of contamination in 2006. The same BCA was used during grape drying, but it showed a limited resistance to available water reduction and little effect on A. carbonarius control. Some differences were observed in musts and wines produced from grapes subjected to different disease control approaches, but further results are necessary for ultimate conclusions. Specific studies were carried out, finalised to multi-fungicide residues detection. Analytic and spectrometric conditions using LC/MS/MS were optimised as well as conditions to conduct toxicity trials. Three cell lines were used to test OTA toxicity: RBL-1, HL-60 and HepG2. The latter was the most sensible to OTA, but difficult to grow, while HL-60 showed an opposite behaviour. RBL-1 was considered the best cell line, as it showed an acceptable susceptibility and was readily grown.
Database assignments for author(s): Paola Battilani, Filippo De Curtis, Vincenzo de Cicco

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
inundative/inoculative release
evaluation - screening - selection

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aspergillus carbonarius	Grapevine (Vitis)	Italy
Aureobasidium pullulans (antagonist)	Aspergillus carbonarius	Grapevine (Vitis)	Italy