Journal of Plant Pathology (2007) 89, S40-S41
P. Ferrante, R. Buonaurio and B.A. Vinatzer (2007)
Phylogenetic characterization of Pseudomonas syringae pv. tabaci and the role played by the effector protein hopq1-1 in determining its virulence and host specificity
Journal of Plant Pathology 89 (3, Suppl.), S40-S41
Poster Presentation at S.I.Pa.V XIV National Meeting - Perugia, Italy 19-21 Sept. 2007
Abstract: Pseudomonas syringae pv. tabaci strains, responsible for wildfire and blackfire diseases of tobacco, were phylogenetically characterized via multilocus sequence typing (MLST). MLST analysis based on the sequence of four gene fragments of eight P. syringae pv. tabaci strains, P. savastanoi pv. phaseolicola 1448A and eleven P. syringae strains already characterized via MLST by Hwang et al. (Appl. Environ. Microbiol. 71: 5182-5191, 2005) revealed that our P. syringae pv. tabaci strains are all closely related to the completely sequenced P. savastanoi pv. phaseolicola 1448A strain. Moreover, we confirmed our previous rep-PCR results that all analyzed Pseudomonas syringae pv. tabaci strains are representatives of the same clonal group. The presence of 21 P. savastanoi pv. phaseolicola 1448A effector genes was determined in the Pseudomonas syringae pv. tabaci strains by PCR. The effector genes hopR1, hopAE1, hopAH2, hopAN1, hopAS1, hopAJ2, hopD1, hopI1, hopX1 and hrpK1 were found. P. syringae pv. tabaci transformants were obtained introducing individually six effector genes of P. savastanoi pv. phaseolicola 1448A which are absent in the P. syringae pv. tabaci genome. Virulence was tested in bean and Nicotiana benthamiana plants. We observed that the growth in planta of the transformant bearing the hopQ1-1 gene was significantly increased in bean and reduced in N. benthamiana. Virulence of the other five transformants was not changed. From our results, we can deduce that hopQ1-1 is an important virulence determinant of P. savastanoi pv. phaseolicola and that it triggers a defence response in N. benthamiana.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Patrizia Ferrante, Boris A. Vinatzer, Roberto Buonaurio
Research topic(s) for pests/diseases/weeds:
molecular biology - genes
identification/taxonomy
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Pseudomonas syringae pv. tabaci | Tobacco (Nicotiana tabacum) | |||
Pseudomonas syringae |