Journal of Plant Pathology (2007) 89, 413-415

L. Tomassoli, A. Zaccaria and A. Tiberini (2007)
Use of one step RT-PCR for detection of Asparagus virus 1
Journal of Plant Pathology 89 (3), 413-415
Abstract: Two viruses are responsible for most of the decline in asparagus crops (Asparagus officinalis L.) in the world: Asparagus virus 1 (AV-1) and Asparagus virus 2 (AV-2). AV-1 is a member of genus Potyvirus and has so far been identified in plant and tissue-culture material of asparagus using biological assays on indicator plants, electron microscopy and, rarely, by serodiagnosis. In this study, degenerate potyvirus primers were used in RT-PCR to amplify a cDNA product from an asparagus potyvirus, previously identified as AV-1 on the basis of host range results. RT-PCR products were sequenced, and the nucleotide sequences obtained were aligned with those of other members of the genus Potyvirus to devise primer pairs specific for AV-1. The use of one primer pair in a one-step RT-PCR procedure was found to yield an amplicon with RNA from an AV-1 infected plant, but not with RNA from plants infected with Turnip mosaic virus (TuMV), Pea seed-borne mosaic virus (PSbMV), or Lettuce mosaic virus (LMV). The method was sensitive and reproducible for the detection of AV-1 in leaves and spears of asparagus samples collected from fields and from tissue-culture plantlets.
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Database assignments for author(s): Laura Tomassoli

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Asparagus virus 1	Asparagus (Asparagus officinalis)