Journal of Plant Pathology (2006) 88, p. S69 (El Maghraby et al.)

I. El Maghraby, J. Sánchez-Navarro, S. Matic, A. Myrta and V. Pallás (2006)
First report of two viruses with filamentous particles from stone fruit trees in Egypt
Journal of Plant Pathology 88 (3, Special Issue), S69-S69
Abstract: A survey was done during spring 2006 in several stone fruit tree orchards of Egypt for infections by Apricot latent virus (ApLV) and Plum bark necrosis stem pittingassociated virus (PBNSPaV). The orchards were in the new reclaimed and old lands of the governorates of Fayoum, Qalyoubia, Giza, Menoufia, Bechira and Behaira. Samples from 29 apricots, 5 European plums, and 5 peaches were collected randomly for RT-PCR analysis. RNA extracted from leaves was used in a Superscript III one-step RT-PCR with Platinum Taq DNA polymerase kit (Invitrogen, Carlsbad, California, USA). A duplex RTPCR was done using sets of specific primers as described by Sánchez-Navarro et al. (2005). Fragments of amplified DNA of 717 or 270 bp, were derived from RNA of ApLV and PBNSPaV, respectively. RT-PCR results were confirmed by uniplex RT-PCR using other primer sets specific for ApLV (Nemchinov and Hadidi, 1998) or PBNSPaV (Amenduni et al., 2005). Of the tested samples, 4 plums and 2 peaches were infected by PBNSPaV, an apricot was infected by ApLV, and 2 plums were infected by both viruses. These infections were not associated with any particular field symptoms. All infected trees belonged to imported cultivars, except for a native apricot cv. Balady, that was infected with ApLV. To our knowledge, this is the first report of infections by ApLV and PBNSPaV in Egypt.
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Database assignments for author(s): Arben Myrta, Vicente Pallas, Slavica Matic

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Apricot latent virus	Apricot (Prunus armeniaca)	Egypt
	Apricot latent virus	Plum/prune (Prunus domestica)	Egypt
	Plum bark necrosis stem pitting-associated virus	Peach/nectarine (Prunus persica)	Egypt
	Plum bark necrosis stem pitting-associated virus	Plum/prune (Prunus domestica)	Egypt