Journal of Plant Pathology (2006) 88, p. S47 (Lanzoni et al.)

C. Lanzoni, C. Ratti, M. Turina, A. Pisi, P. Tedeschi and C. Rubies Autonell (2006)
Molecular characterisation of Allexiviruses from garlic in Italy
Journal of Plant Pathology 88 (3, Special Issue), S47-S47
S.I.Pa.V XIII National Meeting - Foggia, 12-16 September 2006 - Poster
Abstract: Most garlic plants grown in the world are infected with various virus species, including members of the genera Potyvirus, Carlavirus, Fijivirus and Allexivirus, which give rise to mosaic or streak symptoms and reduction of the size of bulbs and cloves. During 2005 and 2006, garlic plants were collected from different provinces of Italy (Ferrara, Rovigo, l'Aquila, Imperia). ssRNAs were extracted from leaf tissues and tested by RT-PCR. Eight samples resulted positive when tested using allexivirus generic primers pair. Nucleotide sequence analysis of PCR products (554 bp) showed the presence of Garlic virus C (GarV-C) and Garlic virus D (GarV-D) infection. To confirm these results, specific primers for coat protein (CP) amplification of GarV-C and GarV-D were designed and complete nucleotide sequence (780 and 753 bp respectively) determined by RT-PCR. The nucleotide sequences identity among our isolates varies between 56.0 and 99.1%. On the basis of deduced CP amino acid sequences, high homology (97.2 to 99.6%) was observed between a Korean isolate of GarV-D (accession numner AF519572) and seven Italian isolates from Ferrara and Rovigo. One sample, collected from Imperia, showed the highest homology (95.4%) with a Japanese isolate of GarV-C (accession number AB010302). Low amino acid sequence identity, ranging from 49.6 to 77.6%, was observed among Italian isolates and others allexivirus species. These results provide experimental evidence of the occurrence of GarV-C and GarV-D in the garlic accessions tested. To our knowledge this is the first molecular characterization of these allexivirus species in Italy.
Database assignments for author(s): Concepcion Rubies Autonell, Massimo Turina

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Garlic virus D	Onion/garlic/leek (Allium)	Italy
	Garlic virus C	Onion/garlic/leek (Allium)	Italy