Journal of Plant Pathology (2006) 88, p. S19 (Ciuffo et al.)
M. Ciuffo, D. Pacifico, V. Masenga and M. Turina (2006)
A new species of tospovirus from Piedmont, Italy
Journal of Plant Pathology 88 (3, Special Issue), S19-S19
S.I.Pa.V XIII National Meeting - Foggia, 12-16 September 2006 - Oral Presentation
Abstract: During surveys of weeds in the vineyards in Piedmont carried out in order to identify possible reservoirs of infection for the phytoplasma disease Bois Noir, Polygonum convolvolus plants showing ringspot symptoms were observed. Electron microscopy of homogenates of these plants showed the presence of a virus with the morphological features of a tospovirus. The viral isolate (called Plg3) was mechanically transmitted to several host plants. Serological tests showed no reaction to any tospovirus so far characterized, with the exception of a very faint reaction in DASELISA with an Iranian isolate of Tomato yellow fruit ring virus (TYFRV). We purified nucleocapsids from Plg3-infected Nicotiana benthamiana and, running these in SDS-PAGE, detected a difference from TSWV isolates in the coat protein size. An antiserum against purified nucleocapsids was prepared and used to specifically detect Plg3 by DAS and ACP ELISA and by Western blotting. Lateral flow devices using gold-conjugated antiserum also detected the virus in crude sap extracts. Cloning and sequencing the full length of segment S and part of the M segment of Plg3 allowed comparison with tospovirus sequences in the databases, indicating that Plg3 is a new tospovirus species most closely related to TYFRV and Iris yellow spot virus (IYSV). Transmission experiments with thrips species in a controlled environment are currently under way.
Database assignments for author(s): Massimo Turina, Davide Pacifico
Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Orthotospovirus (genus) | Italy | |||
| Polygonum convolvulus (weed) | Grapevine (Vitis) | Italy |
