Journal of Plant Pathology (2005) 87, 292-293

From Pestinfo-Wiki
Jump to: navigation, search

S. Davino, A. Lombardo, M. Davino, A. Bertaccini and M.G. Bellardi (2005)
Cucumber mosaic virus infecting Thevetia nereifolia and Nandina domestica
Journal of Plant Pathology 87 (4), 292-293
XII Meeting, Italian Society for Plant Pathology, Reggio Calabria, 29/9 - 1/10, 2005 (poster)
Abstract: In June 2004, virus-like symptoms were observed in the foliage of yellow oleander (Thevetia nereifolia Juss. ex Steud.; family Apocynaceae) and common nandina (Nandina domestica Thunb.; family Nandinaceae) growing in the Botanical Garden of the University of Bologna (Italy). Yellow oleander showed distortion, malformation and chlorotic mottling of younger leaves, stunting, and reduction in the number of flowers; common nandina exhibited narrowed leaves. Symptomatic leaves from both ornamental shrubs were collected and used for mechanical inoculations to herbaceous hosts. Systemic symptoms induced in Nicotiana tabacum "Samsun", N. bethamiana and Capsicum annuum suggested a possible infection by Cucumber mosaic virus (CMV). The presence of this virus was verified by PAS-ELISA using the polyclonal antisera PVAS-30 and PVAS-242a. RT-PCR using primers for CMV movement protein (MP) gene confirmed its presence in yellow oleander and common nandina plants. Both sequences were compared by SSCP assay, and the MP gene was cloned and sequenced. The sequences obtained were compared with those of 16 CMV strains retrieved from GeneBank. Results showed that the two CMV isolates were molecularly similar to each other and closely related to isolate B2 from Musa sapientum. Both belonged to CMV subgroup II (95% nucleotide identity). No records of virus infection to T. nereifolia apparently exist, thus it appears that this ornamental shrub is a new natural host for CMV. As to N. domestica, this is the first record of a natural virus infection of this species in Italy.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Salvatore Walter Davino, Assunta Bertaccini, Maria Grazia Bellardi

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Cucumber mosaic virus Italy
Retrieved from "https://wiki.pestinfo.org/w/index.php?title=Journal_of_Plant_Pathology_(2005)_87,_292-293&oldid=506139"