Journal of Plant Pathology (2004) 86, p. 326 (Myrta et al.)

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A. Myrta, M. Al-Rwahnih, A. Percoco and V. Savino (2004)
Presence of a Plum pox virus recombinant in Apulia
Journal of Plant Pathology 86 (4), 326-326
XI Meeting, Italian Society for Plant Pathology, Milan, 29/9 - 1/10, 2004 (poster)
Abstract: Following successful eradication, no new Plum pox virus (PPV) foci have been detected in Apulia (southern Italy) since 2000, in the course of the region-wide annual monitoring programme implemented since the beginning of 1990s. However, during spring 2003 new PPV infections were discovered in an apricot orchard established at Mesagne (BR), with propagating material coming from an extraregional nursery. Four symptomatic trees (one of cv Ninfa and three of cv San Castrese) were observed, which reacted positively to DASI-ELISA with the universal monoclonal antibody MAb5B. Infected trees were promptly pulled out but studies were initiated for the serological typing the PPV isolate. This was done by DASI-ELISA, using MAb5B and four strain-specific monoclonal antibodies: M-specific (AL), D-specific (4DG5), EA-specific (EA24) and C-specific (AC). Molecular characterization was by RFLP analysis of PCR products targeting two genomic regions, i.e. coat protein (CP) (using RsaI) and P3-6K1 (using DdeI). The isolate proved to be a recombinant as it typed as PPV-M by strain-specific MAbs and RFLP analysis of the CP gene, but as PPV-D in the P3-6K1 gene. Sequence analysis of the (Cter)NIb-(Nter)CP region of the recombinant indicated that the recombination breakpoint was located in the C terminus of the NIb gene, similarly to what reported for other recognized PPV recombinants. This represents the first record of a recombinant PPV isolate in Italy.
Database assignments for author(s): Arben Myrta

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
surveys/sampling/distribution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Plum pox virus Apricot (Prunus armeniaca) Italy
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