Journal of Plant Pathology (2004) 86, p. 315 (Ciuffo et al.)

M. Ciuffo, V. Masenga and M. Turina (2004)
A new potyvirus isolated from Tradescantia zebrina in Piedmont
Journal of Plant Pathology 86 (4), 315-315
XI Meeting, Italian Society for Plant Pathology, Milan, 29/9 - 1/10, 2004 (poster)
Abstract: A new virus provisionally called Tradescantia mosaic virus (TraMV) was isolated from Tradescantia zebrina plants showing mosaic and leaf distortion from a nursery in Piedmont (Northern Italy). Its identification as a new potyvirus species relies on particle morphology, molecular, and serological analysis. The host range was determined through mechanical inoculation: TraMV could systemically infect only T. zebrina reproducing the field symptoms, and caused local lesions in Chenopodium quinoa and C. amaranticolor. Using degenerate primers designed to amplify the carboxy-terminal of the coat protein and the 3' untranslated genomic region a 650 by DNA fragment was amplified by RT-PCR. The product was cloned and sequenced. BLAST search confirmed that TraMV belongs to the genus Potyvirus. The TraMV sequence was aligned with the most closely related potyviral sequences from database using ClustaIX software, and a phylogenetic tree was constructed using Phylip 3.5 software. TraMV was sufficiently unrelated to other potyviruses to be regarded as a putative new species. Based on the sequence two specific primers were designed to be used as a diagnostic tool. RFLP analysis carried out on the RT-PCR product using the restriction enzymes HindIII and PvuII yielded a specific pattern. TraMV did not react in DAS and ACP-ELISA with any of 20 potyviral antisera from the Institute's (IVV-TO) collection.
Database assignments for author(s): Massimo Turina

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tradescantia mosaic virus	Tradescantia (crop)	Italy