Journal of Plant Pathology (2004) 86, p. 314a (Cillo et al.)

F. Cillo and D. Gallitelli (2004)
First characterisation of molecular interactions between Cucumber mosaic virus, its satellite RNA and different Lycopersicon spp. hosts
Journal of Plant Pathology 86 (4), 314-314
XI Meeting, Italian Society for Plant Pathology, Milan, 29/9 - 1/10, 2004 (poster)
Abstract: Several accessions of wild and cultivated species of the genus Lycopersicon, including L. esculentum (tomato), L. hirsutum, L. chilense, L. pimpinellifolium, L. pennelli and L. peruvianum, were tested for their susceptibility to a combination of Cucumber mosaic virus (CMV) with a variant of satellite RNA (satRNA) co-inducing systemic necrosis in tomato. L. hirsutum, L. pennellii and two out of three accessions of L. chilense showed responses to the infection ranging from no symptoms to mild mosaic and leaf malformation, but no necrosis. The following CMV preparations were used for further characterisation of Lycopersicon spp./CMV/satRNA interactions: i) CMV-Fny (no satRNA); ii) CMV-FB (Fny + a Benign satRNA variant, symptomless on tomato); iii) CMV FS (Fny + a tomato Stunting-inducing satRNA variant); iv) CMV-FN (Fny + a tomato Necrosis-inducing satRNA). In tomato, only CMV-FB induced the asymptomatic phenotype that correlated with the down-regulation of viral RNA replication, whereas the other two CMV/satRNA combinations induced severe symptoms and high CMV replication levels. On the contrary, in L. hirsutum all CMV/satRNA combinations suppressed symptoms induced by CMV Fny, and in all cases symptomless infections correlated with the down-regulation of genomic RNA replication. A screening of 99 near isogenic lines, containing single introgressions from L. hirsutum accession LA1777 in the L. esculentum cv E6206 genetic background, is in progress to map host factors determining differential susceptibility to CMV/satRNA in Lycopersicon spp. A first possible resistance factor to stunting, but not to systemic necrosis, has been mapped genetically to L. hirsutum chromosome 6.
Database assignments for author(s): Fabrizio Cillo

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cucumber mosaic virus	Tomato (Solanum lycopersicum)