Journal of Plant Pathology (2003) 85, 292-293

A. Ligorio, L. Schena, F. Nigro and A. Ippolito (2003)
Enzymatic activity and isoform patterns of chitinase and ß-1,3-glucanase in sweet cherries and apricots treated with Aureobasidium pullulans (De Bary) Arnaud, strain L47
Journal of Plant Pathology 85 (4), 292-293
X Meeting, Italian Society for Plant Pathology (poster)
Abstract: Enzymatic activities and isoform patterns of chitinase and ß-1,3-glucanase in sweet cherry and apricot fruits treated with Aureobasidium pullulans, strain L47, were investigated by enzymatic assays and sodium dodecyl sulfate-polyacrilamide gel electrophoresis (SDS-PAGE). Fruits were individually wounded, treated with an aqueous suspension of L47 or sterile distilled water and kept at 24±1°C. At various time intervals (0, 12, 24, 48, 72, 96 h) tissue samples were collected and total protein extracted. Non-wounded fruits served as a control. In sweet cherry and apricot wounds treated with the antagonist, ß-1,3-glucanase activity increased 12 h after treatment, whereas endo- and exochitinase activity increased after 24 h. In untreated wounded fruits a delayed and lower enzymatic activity was observed. In non-wounded control fruits no change in enzymatic activities was found. SDS analysis of extracts from wounded and nonwounded sweet cherry tissues indicated the presence of four constitutive chitinase isoforms; an additional induced isoform, with an apparent molecular mass of 40 kDa, was found in antagonisttreated wounds. Protein extracts from apricots expressed one constitutive isoform of chitinase; another isoform, with an apparent molecular mass of 70 kDa, was observed in L47-treated wounds. Five ß-1,3-glucanase isoforms with an apparent molecular mass similar to those expressed by L47 culture filtrates were observed in sweet cherry and apricot extracts from antagonist-treated wounds.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Leonardo Schena, Antonio Ippolito

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Aureobasidium pullulans (antagonist)