Journal of Plant Pathology (2002) 84, p. 175 (Bertazzon et al.)

N. Bertazzon, E. Angelini and M. Borgo (2002)
Detection of Grapevine leafroll-associated virus-2 (GLRAV-2) by ELISA and RT-PCR
Journal of Plant Pathology 84 (3), 175-175
IX Meeting, Italian Society for Plant Pathology
Abstract: GLRaV-2 is one of the closterovirus associated with leafroll disease of grapevine. The aim of this study was the evaluation of 3 antisera and 4 primer pairs specific for detection of GLRaV-2. About 900 grapevine samples, rootstocks and scions, from Italy and other countries, were collected in 2001-2002. ELISA test showed that only some samples of Italian origin were infected (5.6%), while accessions from abroad of cv. 'Cabernet sauvignon' exhibited a high infection rate, in particular clone 191F (80% infection rate), 337F (55%) and 341 (25%). Comparison among Agritest, Sanofi and Bioreba antisera demonstrated the low reliability of Sanofi antiserum, due to high background values. Antisera furnished by Bioreba and Agritest displayed quite different results, even if antiserum obtained by Agritest was more sensitive. In particular, contrasting results concerned 34% of the samples belonging to the 3 clones of 'C. sauvignon'. RT-PCR were performed with primer pairs LR2-U2/L2, LRaV-2(1)/(2), GLR2CP1/2 and CP96f/r on 33 samples: 15 samples were negative with Bioreba and Agritest antisera, 6 positive with both serological tests and 12 showed contrasting results. Only 7 samples were negative with all primer pairs. The remaining 26 showed positive results with all primer pairs used. In order to evaluate sensitivity of primer pairs, other amplifications were performed on 7 positive samples, after dilution up to 1:10,000 of cDNA extracts. Primers performances were quite similar, even if GLR2CP1/CP2 pairs gave the best results at each dilution.
Database assignments for author(s): Elisa Angelini

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Grapevine leafroll-associated virus 2	Grapevine (Vitis)	Italy