Journal of Plant Diseases and Protection (2016) 123, 19-27

Gul Erginbas-Orakci, Grant Poole, Julie M. Nicol, Timothy Paulitz, Abdelfattah A. Dababat and Kimberley Campbell (2016)
Assessment of inoculation methods to identify resistance to Fusarium crown rot in wheat
Journal of Plant Diseases and Protection 123 (1), 19-27
Abstract: Crown rot, caused by Fusarium culmorum and Fusarium pseudograminearum, is one of the most pervasive diseases of wheat throughout the world. Fusarium culmorum is the most prevalent causal agent in Turkey while F. pseudograminearum is the most predominant in the USA. Consistent and reliable screening methods are required to accelerate the identification and development of wheat cultivars for resistance to Fusarium crown rot in breeding programs. A multifactor experiment with seven replicates was established investigating three different inoculation techniques (seedling dip, stem base droplet and colonized grain) using two pathogenic isolates of F. culmorum in Turkey and Fusarium pseudograminearum in the USA, respectively, against known moderately resistant and susceptible wheat cultivars under controlled greenhouse conditions. Plants were harvested and evaluated for crown rot severity using a 0–10 rating scale. Results indicated that disease severity was greater in seedling dip, compared to colonized grain, and stem base inoculation (in decreasing order of severity), respectively. However, the colonized grain method produced an adequate level of severity and consistent cultivar ranking in both experiments. Results showed significant cultivar × inoculation method interactions, and the two species of Fusarium were considered to be virulent.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Abdelfattah A. Dababat, Timothy C. Paulitz

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium culmorum	Wheat (Triticum)	Turkey
	Fusarium pseudograminearum	Wheat (Triticum)